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EpiQuik Methyl-CpG Binding Domain Protein 2 ChIP Kit P-2017

EpiQuik Methyl-CpG Binding Domain Protein 2 ChIP Kit
P-2017-24 24 reactions  
P-2017-48 48 reactions
Product Overview
 

The EpiQuik™ Methyl-CpG Binding Domain Protein 2 ChIP Kit is a convenient package of tools that allows the experimenter to investigate the binding of MBD2 to methylated DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far superior results than any competitor kits. The EpiQuik™ Methyl-CpG Binding Domain Protein 2 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot as well as DNA microarray. The kit has the following advantages:

  • The fastest procedure available, which can be finished within 5 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple, reliable, and consistent assay conditions.

Principle & Procedure
The EpiQuik™ Methyl-CpG Binding Domain Protein 2 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for MBD2 from mammalian cells. Particularly, this kit includes a ChIP-grade MBD2 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured MBD2 protein-DNA complex, reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.