EpiQuik In Situ Histone H3K27 Tri-Methylation Assay Kit P-3014T

The EpiQuik™ In Situ Histone H3-K27 Tri-Methylation Assay Kit is a convenient package of tools that allows the experimenter to specifically measure histone H3-K27 tri-methylation in situ using cultured adherent cells. The kit has the following advantages:

• Quick and efficient procedure, which can be finished within 3 hours.
• Innovative colorimetric assay without the need for radioactivity, electrophoresis, and chromatography.
• Measurement of in situ histone H3-K27 tri-methylation without the need to prepare cell lysates.
• Microplate format makes the assay suitable for high throughput analysis of agents that increases or inhibits H3-K27 tri-methylation.
• Simple, reliable, and consistent assay conditions.

Principle & Procedure
EpiQuik™ In Situ Histone H3-K27 Tri-Methylation Assay Kit is a whole cell-based detection of tri-methylated H3-K27. In this assay, adherent cells are cultured in conventional 96-well microplates. After your experimental treatment, cells are fixed and permeabilized. The tri- methylated H3-K27 is then detected by an anti-tri-methyl H3-K27 antibody. The ratio or amount of tri-methylated H3 -K27 can be quantified through HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

 
Fig. 1. Schematic procedure for using the EpiQuik In Situ Histone H3K27 Tri-Methylation Assay Kit.

Fig. 2. MDA-231 cancer cells were grown in microplate wells for 48 h and tri-methyl histone H3-K27 was detected with this kit.