Isoprostanes are a group of prostaglandin-like compounds that are produced by the reaction of free radicals with arachidonic acid, including arachidonic esters in phospholipids. Initally reported by Roberts and Morrow as major products of radical mediated lipid oxidation in vivo, they are now widely recognized as reliable biomarkers for oxidative stress. As an average of 50% of isoprostane is excreted as a glucuronide it is important to pre-treat samples with glucuronidase prior to assay. Oxford's ELISA includes glucuronidase as well as a specially formulated buffer allowing direct measurement of isoprostane without the need for expensive SepPaks or immunoaffinity columns. This brings the cost per sample down to 20% of what competing assays cost to run and a fraction of the technician time.
For additional information regarding isoprostane analyses, the influence of isoprostane metabolism, and complete references, see our White Paper.